Gorillas

Trailer for Al Gores An Inconvenient Sequel Truth to Power An Inconvenient Sequel: Truth to Power , Al Gores sequel to his award winning 2006 film, An Inconvenient Truth , is set to be released July 28. The documentary film will explore the progress that has been made since the original film was released and what the future may hold. Check out the trailer below.  download file now

Re analysis of the great Gomez Baena et al dataset They arent messing around! Yesterday I made a short post on this new study in Open Reports.  Ill be honest, I thought it was a seriously impressive sample set, but I was a little skeptical about the results. Not that I didnt believe the authors could find the bacterial proteins, but I was skeptical that the results could be that clean cut. Ive seen a few "identification of digested bacterial proteins in body fluid" studies and even with high resolution data the matrix is complex enough that there is noise there. These results just seemed a little too good to be true. Shoutout to ProteomeXchange/PRIDE and these authors for making this data extremely easy to obtain, sort and reprocess overnight!! For my re-analysis, I threw all the files into PD 2.2 and used the default processing workflow for LTQ-Orbitrap LFQ and LFQ concensus with enhanced annotation.  I made a 77MB FASTA in PD by parsing a UniProt TrembL I downloaded in July...

Reprocessing one of the Bekker Jensen et al datasets! Ummm....so....yesterday I was pretty psyched about the Bekker-Jensen et al., paper in Cell Systems. I pulled all the files from one cell line that I have a LOT of data on. Some Ive fractionated and ran myself. And Ive got nothing that has given me 75% of the PSM/peptide/protein IDs that this does. 33 min nanoLC runs?!? No way Im going to see over 12,000 unique human proteins at high confidence...No way.... I rechecked my setting to make sure I didnt do anything stupid and reran a fractionated dataset from the same cell line from a similar instrument to verify. Nope. This method is legit. Ive just been doing nanoLC the same way for todays super fast instruments that I was doing it for yesterdays not-as -super-fast instruments and inadvertently handicapping the potential they have for 2D fractionated samples! Important disclaimer in my numbers above -- I am using a supplemented FASTA that may be inflating my numbers to some degree (t...