Gorillas

Tube gel digestion (Clicking should expand it!) In-gel digestion appears to be making a comeback in the literature (it works so well, its hard to imagine it ever going away, despite the technical challenges). There is even a spiffy new term for it that eludes me right now that I should add to the translator when I remember. (BTW, I consider the biggest challenges of SDS-PAGE in gel digestion to be time, loading control, reproducibility, enhance sample handling leading to contamination, etc.,) This recent paper suggest a tube-gel digestion protocol that looks like it minimizes a lot of these!  They spike UPS1 protein standards in controlled amounts into a yeast lysate background and compare in-solution, standard in-gel digestion, and their tube based protocol. Label free quan is performed on a quadrupole-Orbitrap and that data is processed in MaxQuant. Not only do they minimize the sample handling, but they appear to retrieve more yeast protein identifications than th...